Strand displacement amplification of RNA targets
US5916779A · kind A · utility
90Cited by
5References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 8, 1997 |
| Grant date | Jun 29, 1999 |
| Priority date | — |
| Expiry date | May 8, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.