Method for differentiation of nucleated red blood cells
US5917584A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 1998 |
| Grant date | Jun 29, 1999 |
| Priority date | — |
| Expiry date | Jan 22, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for differentiation of nucleated red blood cells. In addition, the method provides for a concurrent differentiation of leukocytes in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing nucleated red blood cells in a flow cell by optical analysis. A concurrent differentiation of nucleated blood cells and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method eliminates the use of nuclear stain for identification of nucleated red blood cells. The method of the present invention, for the first time, reports differentiation and enumeration of nucleated red blood cells without using fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.