Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability
US5919445A · kind A · utility
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Key dates
| Filing date | Jun 17, 1997 |
| Grant date | Jul 6, 1999 |
| Priority date | — |
| Expiry date | Jun 17, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N63/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Green fluorescent protein (GFP) gene was transplanted into baculovirus. The baculovirus was used to infect pests of vegetable crops. Three to five days after infection, the infected insects were found to emit strong fluorescence when exposed to a portable UV light. Therefore the spreading of baculovirus infection and disease progression can be effectively detected in the field. In addition, the GFP reduced UV damage to the baculovirus. As a result, GFP can increase the retention time of the virus as a pesticide in the field.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.