Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5919675A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 1995 |
| Grant date | Jul 6, 1999 |
| Priority date | — |
| Expiry date | May 23, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S47/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.