Methods for using a temperature-sensitive plasmid
US5919678A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 17, 1997 |
| Grant date | Jul 6, 1999 |
| Priority date | — |
| Expiry date | Dec 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/902
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the inactivation of a gene in a bacterium or for the introduction of a heterologous gene into a bacterium comprises a) introducing, by transformation or conjugation, in the bacterium a bacterial vector plasmid comprising a marker gene capable of being expressed in a bacterial host strain and an effective replication system which is temperature sensitive at and above a temperature compatible with the viability of the host strain; b) culturing the bacterium on a selective medium at a temperature below the temperature of inhibition to form a culture; c) raising the temperature of the culture to a temperature above the temperature of inhibition; and d) recovering the surviving bacteria after several multiplication cycles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.