Preparation and storage of pancreatic islets
US5919703A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1996 |
| Grant date | Jul 6, 1999 |
| Priority date | — |
| Expiry date | Jun 6, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2509/00
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method includes contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold preservative solution to the warm digest, agitating the warm digest/cold preservative solution at a temperature between about 0.degree. and 15.degree. C., to thereby further digest the partially digested pancreas included in the warm digest, to form cold digest and collecting liquid from the cold digest to form isolated islets. The cold preservative solution and a pancreatic islet preservative solution of the present invention include D-mannitol, K-lactobionate and a buffer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.