Patent · US Expired

Detection and therapy of lesions with biotin/avidin-metal chelating protein conjugates

US5922302A · kind A · utility

99Cited by
6References
36Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 15, 1995
Grant dateJul 13, 1999
Priority date
Expiry dateMay 15, 2015

Classification

  • Technology area (CPC A)Human Necessities
  • CPC primaryA61K2123/00
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Improved methods of detecting and/or treating lesions in a patient are provided. The improved methods comprise the steps of (a) parenterally injecting a subject with a targeting composition comprised of a conjugate of biotin and targeting protein or of an avidin and targeting protein, wherein the targeting protein preferentially binds to a marker substance produced or associated with the targeted lesion, and allowing the targeting protein conjugate to preferentially accrete at the targeted lesion; (b) then parenterally injecting a clearing composition comprised of (i) avidin, when the targeting composition is a biotin-targeting protein conjugate, or (ii) biotin, when the targeting composition is a avidin-targeting protein conjugate, and allowing the clearing composition to substantially clear the targeting composition from non-targeted sites and to bind to the targeting composition accreted at the targeted lesion; (c) parenterally injecting a localization agent which may be the same or different form the clearing agent; (d) parenterally injecting a detection or therapeutic composition comprised of a conjugate of (i) avidin and naturally occurring metal-ion chelating protein chelate…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.