Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
US5922538A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 1996 |
| Grant date | Jul 13, 1999 |
| Priority date | — |
| Expiry date | Dec 12, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method, diagnostic sequences and primers are provided that are useful in identifying the Listeria monocytogenes and Listeria spp. The method involves identifying a RAPD-amplified DNA fragment common to Listeria monocytogenes, then identifying the most conserved regions of that DNA fragment, and the preparing specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all Listeria monocytogenes. Markers within the same fragment that are specific to the Listeria genus are also identified and are useful for the identification of all Listeria spp.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.