Methods for use of mismatch repair systems for the detection and removal of mutant sequences that arise during enzymatic amplification
US5922539A · kind A · utility
106Cited by
1References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 13, 1996 |
| Grant date | Jul 13, 1999 |
| Priority date | — |
| Expiry date | Dec 13, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method for removing DNA molecules containing one or more polymerase-generated mutations in a population of enzymatically amplified DNA duplexes comprising the steps of denaturing and reannealing the population of DNA duplexes, contacting the reannealled DNA duplexes with a mismatch repair system such that each strand is cleaved in DNA duplexes containing a base pair mismatch, and separating the cleaved DNA duplexes from uncleaved DNA duplexes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.