High efficiency gene trap selection of regulated genetic loci
US5922601A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 16, 1996 |
| Grant date | Jul 13, 1999 |
| Priority date | — |
| Expiry date | Sep 16, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A gene trap construct for identification of genes whose activity is regulated upon a cellular transition event which comprises in downstream sequence (i) a cassette having a functional splice acceptor, a translation stop sequence and an internal ribosome entry site and (ii) a promoterless protein coding sequence encoding at least one polypeptide providing positive and negative selection traits. A method for identification of genes whose activity is regulated upon a cellular transition event by introducing the gene trap construct into a cell and observing expression of the positive and/or negative selection traits before and after the transition event.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.