Medium for preserving tissue without tissue culturing occurring
US5925510A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 8, 1997 |
| Grant date | Jul 20, 1999 |
| Priority date | — |
| Expiry date | Oct 8, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/126
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method for autotransplantation, is provided including the steps of (a) obtaining a piece of tissue from one part of a body, (b) preserving the tissue by contacting it with a tissue preserving medium, without tissue culturing occurring, and (c) grafting the tissue in another part of the body. The method is especially advantageous for the repair of hypospadias using buccal mucosa tissue. The tissue preserving medium preferably is a solution of (a) 80-120 mM NaCl, (b) 3-6 mM KCl, (c) 0.1-0.3 mM MgSO.sub.4.7H.sub.2 O, (d) 1-3 mM CaCl.sub.2.2H.sub.2 O, (e) 0.2-0.6 mM NaH.sub.2 PO.sub.4, (f) 1.5-4 mM glucose, (g) 15-25 mM Na lactate, (h) 0.2-0.6 mM Na pyruvate, (i) 0.01-0.05 mM phenol red, (j) 0.1-0.4 mM L-glutamine, (k) 2-35 mM sodium bicarbonate, (l) 125-200 units/ml penicillin-G, (m) 40-60 .mu.g/ml streptomycin sulfate, (n) vehicle consistent with tissue preservation; said medium having a pH ranging from 7.0-7.8.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.