Method for discovery of peptide agonists

US5925529A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Shaun R. Coughlin · San Francisco, US
• Ji Chen · San Francisco, US
• Harold Bernstein · San Francisco, US
• Maki ISHII · Kakegawa, JP
• Ling Wang · Seattle, US
• Mian CHEN · Beijing, CN

Key dates

• Filing date: Jun 7, 1995
• Grant date: Jul 20, 1999
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/974
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention relates to peptide ligand discovery and is particularly directed to a method for the discovery of agonists for membrane bound receptors. The inventive detection system involves the use of a "tethered" ligand for probing receptor binding. The general detection system includes a membrane, a membrane bound receptor, and a chimeric ligand presenting molecule. This chimeric protein forms the tethered ligand and in turn includes a membrane domain, a linker domain, a ligand domain, and a cleavable terminal domain. The "ligands" of the system are exposed by the addition of a specific peptidase that cleaves at the designated sequence. The sequence of the ligand that produces signal as a result of the interaction between the agonist and receptor can be then be isolated using sib selection.