Phagemids and process of preparation

US5925559A · kind A · utility

Assignee

• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• John D. Collins
• Peter Roettgen · Braunschweig, DE

Key dates

• Filing date: Jun 23, 1997
• Grant date: Jul 20, 1999
• Expiry date: Jun 23, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a collection of phagemids made up of a promotor, a gene of a fusion between a ligand protein, wherein the ligand protein in its processed state would be of from 40 to 60 amino acid residues, and a bacteriophage protein, at least one transcription terminator sequence, a replication origin derived from a single strand bacteriophage, a plasmid replication origin and facultatively at least one selection marker. Further, the invention concerns a collection of Escherichia coli clones or cells representing the collection of phagemids, a process for isolating phagemid particles with strong binding characteristics for a defined target molecule with the use of collections according to the invention and phagemid particles obtained according to the process.