Production of trehalose in plants
US5925804A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 1995 |
| Grant date | Jul 20, 1999 |
| Priority date | — |
| Expiry date | Dec 21, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8245
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nucleic acid having (i) a DNA molecule which, when expressed in a plant or plant cell, increases the trehalose content of the plant or plant cell, the DNA molecule encoding an E. coli trehalose phosphate synthase, and (ii) a plant expressible promoter operatively coupled to the DNA molecule. Also, a method for obtaining a plant with increased trehalose production by introducing into a recipient cell of a plant, a plant expressible gene which, when expressed in a plant or plant cell increases the trehalose content of the plant or plant cell. The plant expressible gene is an E. coli trehalose phosphate synthase gene which is operably linked to: a) a transcriptional initiation region that is functional in the plant, and b) a DNA molecule encoding a selectable marker gene that is functional in the plant. The method includes a step of regenerating a plant from the recipient cell under conditions that allow for selection for the presence of the selectable marker gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.