Method for preparing mutant genes
US5928866A · kind A · utility
6Cited by
0References
3Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 15, 1995 |
| Grant date | Jul 27, 1999 |
| Priority date | — |
| Expiry date | Dec 15, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/102
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function to transform the host cell, and culturing the transformant cell in a condition that any mutations of the inserted gene is detectable. According to the present invention, diverse mutant genes can be prepared in a short period of time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.