Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
US5928888A · kind A · utility
32Cited by
28References
45Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 26, 1996 |
| Grant date | Jul 27, 1999 |
| Priority date | — |
| Expiry date | Sep 26, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides for a methods and compositions for identifying proteins or compounds that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting a BL (beta-lactamase) expression construct into an eukaryotic genome, usually non-yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.