Method for expressing recombinant genes in bacteria in absence of antibiotic selection
US5928903A · kind A · utility
1Cited by
1References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1995 |
| Grant date | Jul 27, 1999 |
| Priority date | — |
| Expiry date | Mar 14, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for the expression of recombinant molecules in bacterial hosts in a defined medium in the absence of antibiotic selection. The method uses an expression vector comprising a regulatable promoter by which the production of foreign proteins may be controlled during the growth phase of the culture, an origin of replication maintaining medium vector copy number and a transcriptional terminator.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.