System for real time detection of nucleic acid amplification products
US5928907A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1996 |
| Grant date | Jul 27, 1999 |
| Priority date | — |
| Expiry date | Dec 2, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6484
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative ind…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.