Preparation of a hydrophobic polymer matrix containing immobilized enzymes

US5928918A · kind A · utility

Assignee

• AVL Medical Instruments · CH

Inventors

• Helmut Offenbacher · Graz, AT
• Bernhard Schaffar · Graz, AT
• Massoud Ghahramani · Semriach, AT

Key dates

• Filing date: Apr 14, 1997
• Grant date: Jul 27, 1999
• Expiry date: Apr 14, 2017

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Biological components such as enzymes are immobilized in a three-dimensional cross-linked hydrophobic polymer. An enzyme is mixed with an aqueous solvent and a non-crosslinked prepolymer having an essentially nonpolar main chain with attached polar hydrophilic groups and cross-linking groups. The resultant mixture is exposed to cross-linking temperatures to react the prepolymer via the cross-linking groups without additional catalyst or cross-linking agents, and the aqueous solvent is evaporated to form a three-dimensional cross-linked hydrophobic polymer matrix containing the enzyme. The prepolymer can be an oil alkyl resin containing the main chain with attached polar hydrophilic groups and cross-linking groups. The oil alkyl resin is cross-linked by autoxidation, and aqueous solvent is evaporated to form the polymer matrix. A biosensor is prepared having the enzyme-containing polymer matrix on the surface of a supporting layer which contains cross-linking groups that improve adhesion between the supporting layer and the polymer matrix. Catalytic particles or charge conducting particles may be in the polymer matrix, and multiple polymer matrixes with different enzymes may be form…