Preparation of a cell culture substrate coated with poly-D-lysine
US5932473A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 1997 |
| Grant date | Aug 3, 1999 |
| Priority date | — |
| Expiry date | Sep 30, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cell culture substrate is coated with a composition containing a cell adhesion promoter in a salt solution. A substrate such as plastic, glass or microporous fibers is coated with a composition containing about 5 .mu.g/ml to about 1000 .mu.g/ml of poly-D-lysine in an 0.005 M to about 0.5 M citrate or sulfate salt solution to provide about 50 .mu.l to about 500 .mu.l of the composition per cm.sup.2 of substrate, and the coated substrate is rinsed to remove extraneous materials and dried to obtain a coated substrate having increased shelf-life and/or stability. The coated substrate may be sterilized by rinsing with a sterilizing medium such as ethanol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.