Process for micro biological production of proteins
US5935815A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 1997 |
| Grant date | Aug 10, 1999 |
| Priority date | — |
| Expiry date | May 29, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/60
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention is based on the finding that furin belongs to a family of endoproteolytically active enzymes and relates to a process in the in vitro cleavage of a protein by treating the protein in the presence of Ca.sup.2+ ions with furin, or an endoproteolytically active fragment, derivative or fusion protein of furin. The invention can be used for the (micro) biological production of a protein by culturing genetically engineered cells expressing a pro-form of the protein as well as furin and isolating the protein formed. The invention also relates to a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers, diluents or adjuvants, as well as an endoproteolytically active amount of furin, or a fragment or derivative of furin having an endoproteolytic activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.