Detection of nucleic acid hybrid variation which interacts with double helix or with second reagent through double helix by charge transfer and probe for hybridizing with target nucleic acid
US5939256A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 19, 1996 |
| Grant date | Aug 17, 1999 |
| Priority date | — |
| Expiry date | Jul 19, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting a nucleic acid hybrid comprises steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid, wherein the step for detecting the double helical structure comprises incorporating, into the sample solution, two or more kinds of reagents which are capable of causing a detectable change by interaction therebetween through the double helical structure and measuring the change caused by the interaction of the reagents; and at least one of the two or more kinds of reagents is joined to the probe.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.