Patent · US Expired

Method for capturing a nucleic acid

US5939261A · kind A · utility

14Cited by
4References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 24, 1997
Grant dateAug 17, 1999
Priority date
Expiry dateJun 24, 2017

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6834
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to a method for capturing a class of nucleic acids comprising (a) providing a population of nucleic acids, wherein the population comprises the class; (b) binding a probe moiety to nucleic acids of the class, thereby forming one or more complexes, wherein the probe moiety is attached to a substrate; and (c) capturing the complex. The method can be implemented in certain embodiments in a structure comprised of one or more reservoirs, preferably wherein the reservoirs include one or more second chambers that are in communication with a first chamber. Preferably, the present invention is used to capture the class of nucleic acids that comprise regulatory elements included in a population of nucleic acids isolated from a cell, tissue, or organism. An alternative preferred embodiment of the present invention involves the capture of the class of nucleic acids that comprises certain repetitive elements included in a population of nucleic acids derived from an organism.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.