Hybrid ribozymes and methods of use
US5942395A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | May 9, 1997 |
| Grant date | Aug 24, 1999 |
| Priority date | — |
| Expiry date | May 9, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
New hybrid ribozymes that exhibit the high metabolic stability of small nucleolar ribonucleic acids (snoRNAs), and that cleave target sequences with high efficiencies in either cis (intra-molecular) or trans (inter-molecular) configuration are described. The hybrid ribozymes include (i) a ribozyme catalytic sequence; and (ii) a snoRNA stabilizing motif; wherein the catalytic sequence and the stabilizing motif are arranged to provide the RNA molecule with a three-dimensional configuration in which the catalytic sequence is positioned to interact with a target sequence and the stabilizing motif adopts a conformation that stabilizes the RNA molecule, and additionally enables the RNA molecule to localize in the nucleolus of a cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.