Assays for detecting .beta.-secretase
US5942400A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1996 |
| Grant date | Aug 24, 1999 |
| Priority date | — |
| Expiry date | Jun 7, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/961
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compositions comprising a novel protease capable of cleaving .beta.-amyloid precursor protein (APP) on the amino-terminal side of the .beta.-amyloid peptide therein are provided. The protease is designated .beta.-secretase. Reaction systems comprising .beta.-secretase may be used in screening assays to monitor .beta.-secretase modulated cleavage of APP and to identify .beta.-secretase inhibitors, wherein the .beta.-secretase is in the presence of a suitable polypeptide substrate and cleavage of the substrate determined in the presence and absence of the test substance. Antibodies are raised against peptides of .beta.-secretase. Pharmaceutical compositions and methods comprise compounds identified by screening assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.