Process for preparing a universal support for the synthesis of oligonucleotides
US5942608A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 31, 1997 |
| Grant date | Aug 24, 1999 |
| Priority date | — |
| Expiry date | Mar 31, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process for preparing oligoribo- or oligodeoxyribonucleotides comprising treating an alkanediol or alkanetriol of formula I ##STR1## wherein R.sup.1 =H--(CH.sub.2).sub.n --; and PA1 R.sup.2 =--CH.sub.2 OH or --(CH.sub.2).sub.n --H PA1 n=1-4; with 4,4'-dimethoxytrityl chloride to generate a monosubstituted tritylated compound of formula II ##STR2## wherein R.sup.3 =H--(CH.sub.2).sub.p --; and PA1 R.sup.4 =-CH.sub.2 OH or --(CH.sub.2).sub.p --H; PA1 R.sup.5 is 4,4'-dimethoxytrityl and concommitantly R.sup.6 is hydrogen, or PA1 R.sup.5 is hydrogen and concommitantly R.sup.6 is 4,4'-dimethoxytrityl; PA1 p=1-4; and treating the compound of formula II with one equivalent of a homobifunctional alkanedioic acid halide, and contacting the resulting mixture with a polymer support bearing hydroxyl or aminoalkyl functionalities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.