Method for producing heterologous bi-specific antibodies

US5945311A · kind A · utility

Assignee

• GSF FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT GMBH · DE

Inventors

• Horst Lindhofer · Puchheim, DE
• Stephan Thierfelder · Eichenau, DE

Key dates

• Filing date: Nov 29, 1996
• Grant date: Aug 31, 1999
• Expiry date: Nov 29, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K16/468
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a method for producing heterologous bi-specific antibodies, a quadroma is provided which is fused from hybridromas one of which generates antibodies that have an affinity to the binding site of protein A and another of which generates antibodies that have a weaker or no affinity to the binding domain of protein A, by multiplying and cultivating the quadromas and by eluting the bi-specific antibodies in a pH range at least 0.5 units above the pH value at which the antibodies with greater affinity to the binding domain of protein A are still bonded.