Adenovirus helper-free system for producing recombinant AAV virions lacking oncogenic sequences
US5945335A · kind A · utility
22Cited by
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21Claims
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Assignee
Inventor
Key dates
| Filing date | Jul 16, 1998 |
| Grant date | Aug 31, 1999 |
| Priority date | — |
| Expiry date | Jul 16, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/008
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Composition and methods are provided for producing recombinant AAV ("rAAV") in the absence of helper virus, such as adenovirus. The compositions provide the accessory functions necessary for supporting rAAV virion production in host cells. In certain embodiments, the accessory functions are provided by vectors comprising nucleotide sequences from an adenoviral E4 region which lack the putatively oncogenic E4 ORF 6 coding region. The present invention also includes host cells transfected by the claimed accessory function vectors, methods of using such vectors, and rAAV virions produced by such methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.