Primer for gene amplification, method for nucleic acid discrimination with the use of the same, and nucleic acid discrimination kit
US5948618A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1997 |
| Grant date | Sep 7, 1999 |
| Priority date | — |
| Expiry date | Nov 24, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nucleic acid differentiation method utilizing complementary strand-exchange reaction in competitive hybridization can clearly differentiate a site of gene mutation or a difference between genes in a target nucleic acid even when the gene mutation or difference is located near the primer binding site of the target nucleic acid. The invention provides a method and assay kit for differentiating the identity between two nucleic acids by labeling a gene amplifying primer including a primer main region which is complementary to the target nucleic acid to be amplified and having added to the 5' end of the primer main region a sequence which is noncomplementary to the target nucleic acid; amplifying one of two nucleic acids to be determined for identity using the labeled primer, thereby obtaining a labeled DNA; mixing the labeled DNA with an unlabeled DNA prepared from the other nucleic acid to effect competitive hybridization; and measuring the degree of complementary strand-exchange that has occurred between the labeled DNA and the unlabeled DNA by utilizing the label.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.