Reverse two-hybrid system employing post-translation signal modulation
US5948620A · kind A · utility
21Cited by
2References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 4, 1997 |
| Grant date | Sep 7, 1999 |
| Priority date | — |
| Expiry date | Aug 4, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to modified two-hybrid systems, in particular a reverse two-hybrid system which employs as a reporter a gene encoding a modifying agent such as an enzyme, and a signal agent which is modified by the enzyme usually by being broken down, such that in the event of an inhibition of binding of the two hybrid proteins a detectable signal is produced. The system is particularly useful for drug screening.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.