Host-vector system which can be used in gene therapy
US5948675A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 4, 1996 |
| Grant date | Sep 7, 1999 |
| Priority date | — |
| Expiry date | Oct 4, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2810/855
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates to a system for expressing a transgene in a target cell or a human or animal cell, characterized in that it consists of a eukaryotic cell established as a line, into which there have been transfected: PA1 a) a recombinant viral sequence in which a gene has been deleted totally or partially and substituted by the transgene at the level of this gene; PA1 b) a nucleic acid sequence including a sequence encoding the deleted protein, which sequence is in dependence on a promoter and is combined, where appropriate, with the transgene, and flanked at its 3' end a polyadenylation site; the recombinant viral genome and the sequence, carried by one or two plasmid supports, being capable of trans-complementing each other and allowing the host cell to produce defective infectious viruses.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.