Reagent, method, and kit for the quantitation of oxidation-reduction phenomena in proteins and peptides
US5952186A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 16, 1996 |
| Grant date | Sep 14, 1999 |
| Priority date | — |
| Expiry date | Apr 16, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/182
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A first embodiment of the method is for analyzing the amount of methionine sulfoxide in a protein sample and includes the steps of contacting a protein solution with methionine sulfoxide reductase in the presence of a reducing reagent bearing a covalently-linked reporter tag, whereby the reducing reagent is oxidized. The oxidized reducing reagent formed, which is in proportion to the amount of methionine sulfoxide in the sample, is then quantified. A second embodiment of the method is for analyzing the amount of disulfide linkages in a polypeptide or protein sample. It proceeds in the same fashion as above, but in the absence of any enzyme. A novel fluorescently-labeled reducing agent, and kits to practice the method are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.