Patent · US Expired

Method for separating unmodified hemoglobin from cross-linked hemoglobin

US5952470A · kind A · utility

2Cited by
17References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 7, 1995
Grant dateSep 14, 1999
Priority date
Expiry dateJun 7, 2015

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K14/805
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method for separating unmodified hemoglobin from cross-linked hemoglobin in a hemoglobin solution. The method involves contacting the hemoglobin solution with a least one dissociating agent to form a dissociation solution wherein unmodified tetrameric hemoglobin is dissociated to form hemoglobin dimers. The hemoglobin dimers are then separated from the dissociation solution, while retaining the cross-linked hemoglobin in the dissociation solution.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.