Sequence specific DNA binding by p53

US5955263A · kind A · utility

Assignees

• THE JOHNS HOPKINS UNIVERSITY · US
• Genzyme Corporation · US

Inventors

• Bert Vogelstein · Baltimore, US
• Kenneth W. Kinzler · Baltimore, US
• Michael I. Sherman · Glens Falls, US

Key dates

• Filing date: Sep 1, 1994
• Grant date: Sep 21, 1999
• Expiry date: Sep 1, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Specific sequences in the human genome are the sites of strong binding of wild-type p53 protein, but not mutant forms of the protein. These sequences are used diagnostically to detect cells in which the amount of wild-type p53 is diminished. The sequences can also be used to screen for agents which correct for loss of wild-type p53 to DNA in cancer cells.