Biomaterial immobilization on an Si.sub.3 N.sub.4 surface containing Si-NH.sub.2 groups with a heterobifunctional cross-linking agent

US5955335A · kind A · utility

Assignee

• Foschungszentrum Julich GmbH · DE

Inventors

• Marion Thust · Neunburg vorm Wald, DE
• Michael Schoning · Jülich, DE
• Joachim Vetter · Offenbach, DE
• Ulrich B. Kaupp · Aachen, DE
• Peter Kordos · Jülich, DE
• Hans Luth · Aachen, DE

Key dates

• Filing date: Apr 7, 1997
• Grant date: Sep 21, 1999
• Expiry date: Apr 7, 2017

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Biomaterial such as an enzyme, cell, antigen or antibody is immobilized by covalent bonding to a substrate having an Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups that provide reactive NH.sub.2 groups. A heterobifunctional cross-linking agent having an NH.sub.2 -reactive group and a biomaterial-reactive group is reacted with the NH.sub.2 groups of the surface and then with the biomaterial or the heterobifunctional cross-linking agent is reacted with the biomaterial and then with the NH.sub.2 groups of the surface. The Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups can be formed by precipitating on a substrate a 10-1000 nm thick Si.sub.3 N.sub.4 layer from a SiH.sub.4 /NH.sub.3 mixture, and providing reactive NH.sub.2 groups on the surface of the layer by subjecting the surface to hydrolyzing cleaning.