Method for the determination of mutant restriction enzymes
US5955369A · kind A · utility
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1References
13Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 16, 1997 |
| Grant date | Sep 21, 1999 |
| Priority date | — |
| Expiry date | Jul 16, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/131
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for the determination of mutant restriction enzymes which comprises incubating restriction enzymes under non-native conditions with a labeled double stranded oligonucleotide to a solid support to form an enzyme-oligonucleotide complex and detecting the label to determine cleavage of the oligonucleotide by mutant enzymes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.