Patent · US Expired

Detection of mutation by resolvase cleavage

US5958692A · kind A · utility

123Cited by

6References

53Claims

0Family size

Assignee

Variagenics, Inc. · US

Inventors

Richard G. H. Cotton · Melbourne, AU
Rima Youil · Melbourne, AU
Borries Kemper · Neunburg vorm Wald, DE

Key dates

Filing date	Sep 2, 1997
Grant date	Sep 28, 1999
Priority date	—
Expiry date	Sep 2, 2017

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/156
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.