Method of detecting biologically active substances by using green fluorescent protein
US5958713A · kind A · utility
79Cited by
2References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1997 |
| Grant date | Sep 28, 1999 |
| Priority date | — |
| Expiry date | Mar 14, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9121
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.