Method for quantitative measurement of an enzyme linked immunosorbent assay
US5958715A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 23, 1997 |
| Grant date | Sep 28, 1999 |
| Priority date | — |
| Expiry date | Dec 23, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method of quantitative analysis of an enzyme linked immuno assay comprising the steps of forming an antigen--antibody complex between an antigen present in a sample to be tested and an enzyme labelled antibody and thereafter adding a substrate composition which can be converted into a differently colored product composition by the catalytic action of the enzyme. Quantification is achieved by measuring the time elapsed from addition of the substrate composition until a sudden color change discernible to the eye occurs. The measured time is then compared against a predetermined standard to determine the amount of antigen present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.