Use of recombinant inhibitor from Erythrina caffra for purifying serine proteases

US5958722A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulrich Kohnert · Habach, DE
• Anne Stern · Penzberg, DE
• Stephan Fischer · Weilheim, DE

Key dates

• Filing date: Sep 13, 1996
• Grant date: Sep 28, 1999
• Expiry date: Sep 13, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/815
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Process for purifying serine proteases from a protein mixture by binding the serine protease to an immobilized polypeptide with the activity of an inhibitor DE-3 from Erythrina caffra, removing unbound components from the protein mixture, detaching the serine protease from the inhibitor and separating the immobilized inhibitor from the soluble serine protease and isolating serine protease which is characterized in that a polypeptide is used as the polypeptide which is the product of a prokaryotic or eukaryotic expression of an exogenous nucleic acid. This inhibitor is distinguished by an improved specific activity and is particularly suitable for the purification of plasminogen activators such as tissue plasminogen activators (t-PA and derivatives).