Recombinant DNA coding for signal peptide, selective interacting polypeptide and membrane anchoring sequence

US5958736A · kind A · utility

Assignee

• PIERRE FABRE MÉDICAMENT · FR

Inventors

• Stefan Ståhl · Stockholm, SE
• Per Nygren · Helsingborg, SE
• Marianne Hansson · Sandviken, SE
• Mathias Uhlen · Uppsala, SE
• Thien Nguyen · Saint-Julien-en-Genevois, FR

Key dates

• Filing date: Apr 8, 1996
• Grant date: Sep 28, 1999
• Expiry date: Apr 8, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/883
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Tripartite recombinant DNA encoding fusion proteins which comprise three sequences, i.e., a signal peptide which is operable in a Gram positive bacterium, an immunogenic polypeptide linked thereto which is not normally expressed in a Gram positive bacterium, and a cell wall spanning and a membrane anchoring sequence, as well as their use in Gram positive bacteria to express the resultant fusion protein on their surface are described. The preferred cell wall spanning and anchoring polypeptides include Staphylococcus protein A and Streptococcus protein G.