Polysaccharides having a high iduronic acid content
US5958899A · kind A · utility
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Key dates
| Filing date | Apr 12, 1996 |
| Grant date | Sep 28, 1999 |
| Priority date | — |
| Expiry date | Apr 12, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08B37/0075
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Process for the preparation of polysaccharides having a high iduronic acid content comprising: PA1 a) N-deacetylation of the polysaccharide K5 from E. coli or of the heparan sulfate or O-desulfation of heparin or heparan sulfate; PA1 b) N-sulfation of the product obtained from the stage a); PA1 c) epimerization in presence of the C5 epimerase enzyme; PA1 d) sulfation of at least some free hydroxy groups, wherein the stage c) is carried out in a reaction medium constituted by a classical buffer solution formed by HEPES, potassium chloride, EDTA and TRITON X-100 to which a suitable additive is added.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.