Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD

US5962253A · kind A · utility

Assignee

• Boehringer Ingelheim International GmbH · DE

Inventors

• Thomas Kupke · Tübingen, DE
• Friedrich Gotz · Tübingen, DE
• Christoph Kempter · Neuhausen ob Eck, DE
• Gunther Jung · Tübingen, DE

Key dates

• Filing date: May 13, 1996
• Grant date: Oct 5, 1999
• Expiry date: May 13, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is described for oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, wherein the peptide comprises at its carboxy terminus the amino acid sequence X.sub.1 X.sub.2 X.sub.3 X.sub.4 X.sub.5 X.sub.6 C, wherein X.sub.1, X.sub.2, X.sub.3 and X.sub.4 are any one of the twenty common amino acids, X.sub.5 is Tyr, Val, Met, Leu, Ile, Phe or Trp, and X.sub.6 is Cys, Ala, Ser, Val, or Thr, whereby the oxidative decarboxylation of the peptide occurs.