Replication-competent recombinant viral vaccines and method of producing same

US5965124A · kind A · utility

Assignees

• Whitehead Institute For Biomedical Research · US
• American Cynamid Co. · US

Inventors

• Mark B. Feinberg · San Francisco, US
• Raul Andino · New York, US
• Carolyn L. Weeks-Levy · Honolulu, US
• Patricia Reilly · Glen Rock, US

Key dates

• Filing date: Jan 31, 1995
• Grant date: Oct 12, 1999
• Expiry date: Jan 31, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Replication-competent recombinant viruses, particularly replication-competent recombinant polioviruses, which include (1) exogenous nucleic acid sequences which encode an exogenous polypeptide and (2) a nucleic acid sequence which encodes an artificial proteolytic cleavage site for a viral or cellular protease which proteolytically processes (cleaves) the precursor protein produced by the parent virus and uses therefor. The recombinant precursor is cleaved into the usual array of constituent proteins, freeing the exogenous polypeptide. Replication-competent recombinant viruses are useful as vaccines against bacterial, viral, fungal and yeast infections, parasitic diseases, cancer and allergies.