Antibodies and proteins useful for assaying virus infection in grape plants
US5965355A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 5, 1996 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Sep 5, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/826
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An approximately 37 kilodalton (kd) protein associated with grapevine leafroll disease infected plants is disclosed. The 37 kd protein is the coat protein for a grapevine leafroll-associated virus designated GLRaV-8. The grapevine virus-encoded 37 kd polypeptide is immunologically distinct from the approximately 36 kd proteins associated with GLRaV-4 or GLRaV-5 or the approximately 38 kd protein associated with GLRaV-1. The invention further provides a substantially pure antibody directed against the 37 kd virus-associated protein, a stable cell line capable of producing such a monoclonal antibody, and a method for assaying for a virus infection in Vitis species. The method involves detecting the presence of a 37 kd polypeptide encoded by an RNA-containing plant virus using an antibody that does not react with a viral encoded polypeptide of ca. 38 kd.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.