DNA which regulates gene expression in coryneform bacteria

US5965391A · kind A · utility

Assignee

• Forschungszentrum Jülich GmbH · DE

Inventors

• Dieter J. Reinscheid · Neu-Ulm, DE
• Bernhard Eikmanns · Ulm, DE
• Hermann Sahm · Jülich, DE

Key dates

• Filing date: May 8, 1997
• Grant date: Oct 12, 1999
• Expiry date: May 8, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/88
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns a DNA fragment located in front of the malate synthase gene of a coryne-form bacterium and isolated from the latter. Any structural gene which codes for a protein can be inserted after this DNA fragment. After transformation of such a construct into a coryne-form bacterium, expression of the structural gene inserted after the DNA fragment is regulated. The invention also concerns a process for synthesizing any protein by culturing a transformed coryne-form bacterium. A bacterium of this type contains in replicable form a DNA fragment isolated from the malate synthase gene of a coryne-form bacterium, and after which the structural gene which codes for the protein to be synthesized is inserted. Since expression of the structural gene which codes for the protein to be synthesized is regulated by the DNA located in front of it, the structural gene is expressed and the desired protein synthesized as soon as a suitable inducing agent is added to the medium.