Method for introducing nucleic acids into higher eukaryotic cells
US5965404A · kind A · utility
15Cited by
6References
49Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1997 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Mar 6, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P35/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Process and medium for the transfection of higher eukaryotic cells with DNA/polycation complexes, wherein a medium is used which contains ethyleneglycol and/or glycerol. The medium may also contain a substance which prevents the acidification of the endosomes, and/or a lower alcohol such as ethanol. The process is particularly suitable for the transfection of primary cells such as fibroblasts. Stably transformed cells can be obtained thereby, e.g. tumour cells for use as tumour vaccines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.