Lysophospholipase produced from aspergillus by recombinant methods

US5965422A · kind A · utility

Assignee

• Rohm Co., Ltd. · JP

Inventors

• Fridolin Loffler · Bensheim, DE
• Quoc Khanh Nguyen · Reichelsheim, DE
• Erwin Schuster · Bechhofen, DE
• Bruno Sprossler · Roßdorf, DE
• Lutz Thomas · New Brunswick, US
• Sabine Wolf · Otzberg, DE

Key dates

• Filing date: May 20, 1997
• Grant date: Oct 12, 1999
• Expiry date: May 20, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a recombinant deoxyribonucleic acid (DNA) isolatable from Aspergillus, preferably Aspergillus foetidus, wherein it codes for a lysophospholipase (LPL) and has the nucleotide sequence for mature LPL stated in SEQ ID NO:1 or a nucleotide sequence derived therefrom, which hybridises under stringent conditions with the nucleotide sequence for mature LPL stated in SEQ ID NO:1. The invention also relates to vectors, to transformed host organisms and to processes for the production of LPL. The invention also provides enzyme products for the production of maltose syrup and products produced in this manner.