Strain for the production of 6-demethyltetracycline, method for producing the strain and vector for use in the method
US5965429A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 27, 1998 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Feb 27, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/889
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant S. aureofaciens cells are provided. These cells comprise: (a) at least one CTC 11 gene; and (b) optionally PA1 (i) a CTC 09 gene; PA1 (ii) a CTC 03 gene; or PA1 (iii) a combination thereof; wherein: PA1 the CTC 11 gene is chromosomal, extra-chromosomal, or chromosomal and extra-chromosomal; PA1 the CTC 09 gene, CTC 03 gene, or a combination thereof is chromosomal, extra-chromosomal, or a combination thereof; expression of the CTC 11 gene is enhanced over that of a wild-type S. aureofaciens cell; and PA1 optionally, the CTC 09 gene, the CTC 03 gene, or both of the CTC 09 gene and the CTC 03 gene are inactivated. The present invention also contemplates vector pLP21329 and vectors for allelic replacement in a S. aureofaciens host cell. The vectors comprise: PA1 (a) a functional E. coli origin of replication; PA1 (b) a functional Streptomyces origin of replication; PA1 (c) a functional gene that imparts a positively selectable phenotype on the host cell; and PA1 ((d) a ribosomal S12 gene which is expressed in Streptomyces such that it imparts sensitivity to streptomycin to the host cell. In another embodiment, a method of mutating a target gene of a biosynthetic pathway of …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.