System for in vitro transposition
US5965443A · kind A · utility
289Cited by
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21Claims
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Key dates
| Filing date | Sep 9, 1996 |
| Grant date | Oct 12, 1999 |
| Priority date | — |
| Expiry date | Sep 9, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.